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Table 3 2008 Practice parameter: recommended proficiency testing and quality assurance technique for prick/puncture skin testing[3]

From: Skin prick/puncture testing in North America: a call for standards and consistency

Procedure • Using desired skin test device, perform skin testing with positive (histamine 1–10) and negative controls (saline 1–10) in an alternate pattern on a subject’s back
• Record histamine results at 8 minutes by outlining wheals with a felt tip pen and transferring results with transparent tape to a blank sheet of paper
• Record saline results at 15 minutes by outlining wheal and flares with a felt tip pen and transferring results with transparent tape to a blank sheet of paper
Calculations • Calculate the mean and SDs of each mean wheal diameter
• Determine coefficient of variation (CV) = SD/mean
Quality Standards • Histamine Control: CV less than 30%
• Saline Control: All negative controls should be <3 mm wheals and <10 mm flares