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Figure 1 | Allergy, Asthma & Clinical Immunology

Figure 1

From: Coaggregation of FcεRI with FcγRIIB Inhibits Degranulation but Not Induction of Bcl-2 Family Members A1 and Bim in Mast Cells

Figure 1

Inhibition of IgE-mediated mast-cell degranulation by FcγRIIB. A, Mast cells were sensitized with 0.1 μg/mL of monoclonal anti-dinitrophenyl (anti-DNP) clone SPE-7 immunoglobulin E (IgE) mouse antibody (anti-DNP IgE) before being challenged with 30 μg/mL of rabbit anti-mouse (RAM) F(ab')2 or 45 μg/mL of RAM immunoglobulin G (IgG) for 30 minutes. Resting cells or cells incubated with 0.1 μg/mL of anti-DNP IgE served as a background control. The figure represents mast-cell activation estimated by β-hexosaminidase release as measured by absorbance. B, Mast cells were sensitized with 0.1 μg/mL of monoclonal anti-DNP clone SPE-7 IgE mouse antibody (anti-DNP IgE) and preincubated with or without 5 μg/mL of RAM CD16/CD32 (FcγIII/II receptor) monoclonal antibody (2.4G2 rat Ab), before challenge with 10 μg/mL of TNP-F(ab') [2] mouse anti-rat (MAR) for 30 minutes. Cells incubated with 0.1 μg/mL of anti-DNP IgE or 0.1 μg/mL of anti-DNP IgE and 5 μg/mL of 2.4G2 rat Ab served as a background control (n = 6; results are presented as mean ± standard error of the mean). *p < .05.

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