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Figure 3 | Allergy, Asthma & Clinical Immunology

Figure 3

From: Lymphocyte Responses to Chymotrypsin- or Trypsin V-Digested β-Lactoglobulin in Patients with Cow's Milk Allergy

Figure 3

Evaluation of reduced Bcell epitopes in digested peptides. A, Immunoglobulin (Ig)G inhibition enzyme-linked immunosorbent assay (ELISA). Various amounts of intact β-lactoglobulin (BLG), chymotrypsindigested BLG polypeptides, or trypsin V-digested polypeptides were co-incubated as inhibitors with rabbit anti- BLG antisera in ELISA assay. B, IgE dot blotting. Twenty micrograms of cow's milk, BLG, extensively hydrolyzed BLG (as a negative control), chymotrypsin-digested BLG polypeptides, and trypsin V-digested BLG polypeptides were applied onto nitrocellulose filter paper. Control and patient 2's sera were used as first antibodies in IgE dot blotting.

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Allergy, Asthma & Clinical Immunology

ISSN: 1710-1492

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