Figure 5From: Chitosan Interferon-γ Nanogene Therapy for Lung Disease: Modulation of T-Cell and Dendritic Cell Immune ResponsesExpression of Toll-like receptors (TLRs) on dendritic cells. Total ribonucleic acid (RNA) was purified from CD11c+ cells isolated by magnetic bead separation from peribronchial lymph nodes of mice with or without chitosan interferon-γ nanogene treatment. RNAs were reverse-transcribed and used as a template for reverse transcriptase-polymerase chain reaction (PCR) with primer pairs and PCR conditions specific for the indicated TLRs. The results of PCRs for 25 cycles are shown with β-actin used as a control (one typical experiment of two).Back to article page