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Figure 2 | Allergy, Asthma & Clinical Immunology

Figure 2

From: TSLP directly impairs pulmonary Treg function: association with aberrant tolerogenic immunity in asthmatic airway

Figure 2

TSLP inhibits suppressive activity and IL-10 production by Treg. A. Suppressive activity of un-stimulated vs. TSLP-primed HC pulmonary Treg against Teff proliferation (n = 8) represented as thymidine counts in suppression assays (left) and percentage suppression of Teff proliferation (right). Percentage suppression of Teff proliferation was calculated by percentage decrease in thymidine uptake in co-cultures of Teff and Treg compared to cultures of Teff alone. B. (Top) Representative FACS plots of IL-10 production by PMA/Ionomycin (PMA/I) activated HC pulmonary Treg after being primed with IL-2, IL-7, and TSLP. (Middle) Expression of IL-10 by PMA/I activated HC pulmonary Treg after being primed with different cytokines (n = 7). (Bottom left) Representative FACS plots of IL-10 production by PMA/Ionomycin (PMA/I) activated HC pulmonary Teff after being primed with TSLP. (Bottom right) Expression of IL-10 by PMA/I activated HC pulmonary Teff after being primed with TSLP (n = 7). Data represented intracellular flow cytometric and ELISA results. C. (Top) Effects of exogenous IL-10 on suppressive activity of TSLP-primed HC pulmonary Treg against Teff proliferation (n = 7). (Bottom) Effects of neutralizing antibodies against IL-10 on suppressive activity of HC pulmonary Treg against Teff proliferation (n = 7). Data were represented as thymidine uptake in suppression assay cultures (left) as well as percentage suppression of Teff proliferation (right). Wilcoxon tests were used for statistical analysis. Bar graphs and horizontal bars represented median values as indicated throughout the figure.

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