Figure 1
From: VIP Regulates the Development & Proliferation of Treg in vivo in spleen
VIP Facilitates Thymic Production Of Treg (Helios+Foxp3+CD4+CD25+). In this VIP KO mouse thymus treated with VIP, there were 596.09 Tregs expressing CD4 and CD25. This is in contrast to untreated VIP KO mice (fig. 3) which is associated with fewer Treg. Gated on CD4-CD25+ lymphocytes (which were orange cellsin the figure above, there are more Helios+CD25+FoxP3+CD4- cells in VIP-treated VIP KO mice than seen in untreated VIP KOmice shown in Fig.2 (red circle with arrow).