VIP Regulates the Development & Proliferation of Treg in vivo in spleen

Table 1 Comparisons of T-cell expression in thymus cells among three mice groups

	Group 1: Controls (n = 10)		Group 2: VIP KO (n = 5)		Group 3: VIP KO + VIP (n = 5)		p-values for Group Comparisons
	Median	Min, Max	Median	Min, Max	Median	Min, Max	1 vs. 2	1 vs. 3	2 vs. 3
CD4-CD25+	1146	666, 1831	3298	2953, 3517	3182	2579, 4109	0.0022*	0.0022*	0.47
CD4-CD25+Helios+	308	110, 436	2690	2520, 2966	1558	450, 1783	0.0022*	0.0022*	0.0091*
CD4-CD25+FoxP3+Helios+	3	0, 22	11	2, 15	43	21, 1771	0.18	0.0031*	0.0091*
CD4+CD25+ (Treg)	159	56, 563	204	177, 281	432	377, 596	0.63	0.015*	0.0091*
CD4+CD25+FoxP3+Helios- (Treg induced intrasplenically)	44	0, 493	25	15, 58	48	25, 493	0.81	0.63	0.12
CD4+CD25+FoxP3-Helios+ (Treg)	46	4, 79	141	111, 166	141	23, 171	0.0022*	0.028	0.92
CD4+CD25+FoxP3+Helios+ (Treg)	21	0, 423	25	15, 53	31	16, 378	0.81	0.40	0.26

Note: p-values were based on Mann-Whitney tests; *: statistically significant at adjusted alpha = 0.017.

ISSN: 1710-1492