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Figure 1 | Allergy, Asthma & Clinical Immunology

Figure 1

From: Basophil activation test compared to skin prick test and fluorescence enzyme immunoassay for aeroallergen-specific Immunoglobulin-E

Figure 1

Example of basophil activation assay. Buffy coat cells suspended in an interleukin-3 (IL-3)-containing buffer were stimulated with (a) negative control (Glycerol Saline); (b) positive control (anti-FcεRI) and (c) Timothy allergen. Basophills were defined as cells not expressing CD3, CD8, CD14, CD19 or HLA-DR (P2) and expressing CD123 and IgE (P3). Activated basophils were defined as CD63+ basophils (P4).

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Allergy, Asthma & Clinical Immunology

ISSN: 1710-1492

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