Figure 2

Effects of ASD on goblet cells and subepithelial layer of the airway. (A) No pathological changes in lungs treated with saline for four times. (B) Slight bronchitis in the airway exposed to ASD alone for four times. (C) Very slight proliferation of goblet cells in the airway epithelium exposed to OVA alone for four times. (D) Marked proliferation of goblet cells that have mucus stained pink with PAS solution, slight infiltration of inflammatory cells into connective tissue around the airway and marked fibrous thickening of the subepithelial layer in the main bronchus treated with OVA and ASD for four times. (E) No pathological changes in lungs treated with saline for eight times. (F) Slight proliferation of bronchial cells and bronchitis in the airway exposed to ASD alone for eight times. (G) Mild proliferation of goblet cells in the airway epithelium exposed to OVA alone for eight times. (H) Mild proliferation of goblet cells, moderate fibrous thickening of the subepithelial layer and moderate infiltration of inflammatory cells into connective tissue around the airway co-exposed to OVA and ASD for eight times. (A–H) PAS stain.