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Figure 1 | Allergy, Asthma & Clinical Immunology

Figure 1

From: Ingestion of milk containing the Dp2 peptide, a dust mite allergen, protects mice from allergic airway inflammation and hyper-responsiveness

Figure 1

Schematic map of the αLA-CN-Dp2t transgene construct and detection of its integration into transgenic mice. (A) The structure of the αLA-CN-Dp2t-bGHpoly(A) fusion gene is shown. The construct includes the mammary gland-specific αLA promoter, the αS1-casein (CN) signal peptide leader sequence, the Dermatophagoides pteronyssinus mite truncated Dp2 (Dp2-t) cDNA, and the bovine growth hormone gene polyA signal. This construct was digested with Bam HI and Bbs I for microinjection. A primer pair for PCR screening was designed to flank the junction of the 3′-end of the αLA promoter and the 5′-end of the Dp2t cDNA. (B) Transgenic founder mice were identified through PCR screening. Mr. represents a DNA size marker of the 100-bp leader. (C) Southern blots showing the integration patterns of the transgene in the transgenic mice. PC represents pCR-αLA-CN-Dp2t plasmid DNA (positive control). NC represents normal mouse DNA (negative control).

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