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Figure 2 | Allergy, Asthma & Clinical Immunology

Figure 2

From: Ingestion of milk containing the Dp2 peptide, a dust mite allergen, protects mice from allergic airway inflammation and hyper-responsiveness

Figure 2

Recombinant Dp2 expression in the transgenic mammary gland and secretion in milk. (A) Freshly dissected mammary glands from Dp2-transgenic and wild type mice in the D15 lactating stage were fixed and stained with hematoxylin and eosin (H&E; left panel) and with antibodies for immunohistochemical (IHC; right panel) analysis. The structures of the mammary gland are labeled as ‘MA’ for mammary acini tissues and ‘LT’ for lactiferous tubules or secretary tubules. Samples were viewed using an epifluorescence microscope (original magnification, 100X). (B) Milk from transgenic female mice was collected, centrifuged, and subjected to SDS-PAGE (upper panel). The expression of the recombinant Dp2 protein was detected in this milk by Western blot analysis using a rabbit anti-Dp2 antibody (lower panel). D7 represents the transgenic milk that was collected from transgenic mice on 7th day of lactation (early stage). D14 and D21 represent the transgenic milk collected during the middle lactation stage. D28 represents the transgenic milk collected during the late lactation stage. Mr. represents a low-molecular-weight protein marker. PC represents the sample containing water and purified Dp2. NC represents the milk collected from wild-type mice. (C) Quantification of the amounts of recombinant Dp2 protein in the transgenic milk samples collected during different stages of lactation.

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