Figure 3

Experimental protocol. Transgenic Dp2 milk and wild-type (WT) milk were collected and fed to 3-week-old pups for 28 days. Mice were divided into three experimental groups. Two of the three groups of mice were sensitized by intraperitoneal injection of 10 μg of purified Dp2 on days 29 and 43. All mice were then challenged with 0.1% Dp2 aerosols on day 50. Mice underwent pulmonary function testing 18 h after aerosol challenge. BAL fluid, serum, and lung sections were collected on day 51 for analysis of the level of airway inflammation.