Figure 3

Unfolded protein response activation in cells with ORMDL3 knockdown. ER stress was induced in 1HAE cells by stimulation with A) tunicamycin or B) thapsigargin for 2 or 4 hours. Relative expression levels of XBP-1u, XBP-1s, and CHOP were quantified and compared to unstimulated cells. 1HAE cells with ORMDL3 knockdown were stimulated with C) tunicamycin or D) thapsigargin for 4 hours. Relative gene expression levels were quantified and compared to cells transfected with scramble (a non-specific) siRNA. Data represent the mean ± SEM of three experimental repeats. Statistical analysis was performed using one-way ANOVA with Bonferroni post-test. *** p < 0.001, ** p < 0.01, * p < 0.05. E) Western blot analysis of p-eIF2α with β-actin loading control. Lysates are from unstimulated 1HAE cells 24 hours after ORMDL3 knockdown. Blot is representative of three independent experiments.