Figure 2
From: Therapeutic potential of anti-IL-6 therapies for granulocytic airway inflammation in asthma
IL-6 is produced by allergen-stimulated mononuclear phagocytes and mediates allergic airway inflammation through eosinophil/neutrophil chemokines/cytokines. WT or IL-6 KO mice received 25 μg HDM i.n. daily for 10d, with or without 50 μg anti-IL-6 (αIL-6) or control IgG i.p. on d0, 3, 5 and 7. (A) IL-6 by ELISA in lung homogenates, or cultures of dendritic cells or alveolar macrophages. (B, C) Cell counts from bronchoalveolar lavage (BAL) or digested lung tissue quantified by hemocytometer manual counting with Turks, followed by differential cell counting of Hema 3-stained cytospins or flow cytometry. (D) Cytokines in lung tissue homogenates quantified by Luminex. Mean + SEM, n = 3-5 mice per group per experiment, 2-3 independent experiments performed. *p < 0.05 compared to saline, media, WT HDM, or HDM + IgG control groups. **p < 0.01. ***p < 0.001.