Fig. 1

a Schematic arrangement of immobilized allergen extracts, their recombinant proteins, and marker spots (М) on biological microchips. Each antigen was immobilized in quadruplicate. Non-specific binding was monitored using spots that contain no proteins (empty gel). b An example of fluorescent image of a biochip after immunoassay of a blood serum sample from a patient with allergy to birch pollen and cat dander