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Table 1 Identification of allergens from Lantana camara pollen grains using MALDI TOF/TOF

From: Clinical and immuno-proteomic approach on Lantana camara pollen allergy—a major health hazard

SN

Identified proteind

Organism

Accession no.

Identified peptidesb

E valuea

Expt. MW/pI

Theor. MW/pI

Score

UPb

Seq. cov. (%)

1.

Actin

Glycine max

CAA23728.1

KIWHHTFYNELRV

0.0019

45.6/5.8

41.56/5.23

64

2

3.45

Maurandya

barclaiana

AAL89713.1

KSFELPDGQVITIGNERF

6.2e−05

19.996/5.02

78

9.94

2.

Actin

Glycine max

CAA23728.1

KIWHHTFYNELRV

0.00017

45.6/5.8

41.56/5.23

69

3

3.45

Chlorella vulgaris

AAB07499.1

KSYELPDGQVITIGNERF

1.3e−05

23.18/4.89

83

8.78

Phaseolus vulgaris

AGV54465.1

RTTGIVLDSGNGVSHTVPIYEGYALPHAILRL

0.0037

41.66/5.39

50

8.48

3.

TLP; Ole e 13

Olea Europea

E3SU11.1

RCPDAYSYPQDDPTSLFTCPAGTNYRV

1.3

19.4/4.2

24.7/5.1

31

2

11.94

HP

Erythranthe guttata

EYU38379.1

RCTAPINEQCPNELRA

0.075

 

24.9/5.3

42

6.95

4.

F1ATPase

Pisum sativum

BAA20135.1

KTEHFLPIHRE

0.014

51.8/5.5

60.1/6.6

52

3

1.96

ATP Synthase subunit beta

Nicotiana plumbaginifolia

CAA26620

RFTQANSEVSALLGRI

0.027

59.8/5.9

48

2.85

Tricum aestivum

CAA52636.1

RLVLEVAQHLGENVVRT

0.013

59.24/5.5

50

3.07

5.

ATP Synthase beta subunit

Tricum aestivum

CAA52636.1

RLVLEVAQHLGENVVRT

0.046

51.8/5.6

59.2/5.56

44

2

3.07

Nicotiana plumbaginifolia

CAA26620.1

RDAEGQDVLLFIDNIFRF

0.0077

59.85/5.95

56

3.21

6.

ATP Synthase beta subunit

Triticum aestivum

CAA52636.1

RLVLEVAQHLGENVVRT

0.43

51.84/

59.24/5.56

38

2

3.07

Nicotiana plumbaginifolia

CAA26620.1

RDAEGQDVLLFIDNIFRF

0.29

5.7

59.85/5.95

40

3.21

7.

TLP; Ole e 13

Olea Europea

E3SU11.1

RCPDAYSYPQDDPTSLFTCPAGTNYRV

5.1e−13

21.85/6.5

24.72/5.10

150

3

11.94

Predicted TLP

Solanum Lycopersicum

XP_004252644.1

RLDSGQSWQVNVAARVRAR

NAc

45.53/4.95

 

4.55

PRP

Solanum tuberosum

XP_006364119.1

NQQCPNELRN

NAc

 

25.00/5.31

 

4.4

8.

TLP; Ole e 13

(Olea Europea)

E3SU11.1

RCPDAYSYPQDDPT

SLFTCPAGTNYRV

5.9e−06

19.28/6.159

24.72/5.10

79

2

11.94

HP

Erythranthe guttata

EYU38385.1

RCTAPINEQCPNELRA

0.018

81.11/8.03

48

2.2

9.

Predicted GAD 1- like

Glycine max

XP_003544967.1

KVLHELDLLPARV

0.94

 

52.685/5.831

34

2

2.58

GAD

Nicotiana tabacum)

AAM48129.1

HNEFELSDTLR

NAc

  

2.21

  1. aProbability score at the <0.05 level
  2. bUP, unique peptides have been identified by MASCOT search against NCBI database
  3. cManual de novo sequencing has been performed for the respective peptide sequence
  4. dIdentified proteins: actin, TLP, thaumatin like protein; HP, hypothetical protein MIMGU_ngvla013131 mg; PRP, pathogenesis related protein, GAD, glutamic acid decarboxylase
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Allergy, Asthma & Clinical Immunology

ISSN: 1710-1492

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