Fig. 1
From: Gene therapy for primary immune deficiencies: a Canadian perspective
Ex vivo gene therapy. Patient’s cells are collected from bone marrow, peripheral blood or umbilical cord blood (1). A virus is altered ex vivo to increase safety and efficacy of gene delivery (2). A gene is inserted into the altered virus ex vivo (3). The altered virus containing the gene is added to the patient’s cells ex vivo (4). The cells are genetically altered ex vivo (5). The patient is treated with chemotherapy or radiotherapy (6). The genetically altered cells are transplanted into the conditioned patient (7). The genetically altered cells expand in the patient and exert their biological effects (8)