Fig. 3
From: Regulation of Th2 responses by different cell types expressing the interleukin-31 receptor
IL-31RA−/− mice nasally injected with Cry j 2 show decreased antigen-specific proliferative and Th2 cytokine responses. 7 days after 15 nasal administrations of Cry j 2, spleen cells from WT (IL-31RA+/+, N = 6) and KO (IL-31RA−/−, N = 7) mice were cultured for 88 h in the presence or absence of Cry j 2 (0.1 μg/ml) and pulsed for the last 16 h with 0.5 μCi of tritiated thymidine. The results of T-cell proliferation (a) are expressed in terms of a stimulation index (SI) calculated by dividing counts per minute (cpm) in the presence of antigen with cpm in the absence of antigen. Supernatants obtained from a 72-h cell culture were assayed by ELISA for IL-13 (b) and IL-5 (c). Data are presented as the mean ± SD