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Fig. 1 | Allergy, Asthma & Clinical Immunology

Fig. 1

From: Preconception allergen sensitization can induce B10 cells in offspring: a potential main role for maternal IgG

Fig. 1

Effects of preconception immunization on offspring antibody production, lung inflammation and phenotype. Offspring from immunized or non-immunized mothers were immunized with OVA in the neonatal period and evaluated at 20 days of age [20 (Im)]. The total IgE, anti-OVA IgG1 and anti-OVA IgM levels were determined by ELISA, and anaphylactic IgE was determined by PCA (a). BAL preparation, histological analysis and dissociation of offspring lungs were performed in 43-day-old offspring after five intranasal challenges. The differential cell counts in BAL were evaluated by flow cytometry (b). Histological examinations were performed on samples stained with H&E (c). The dissociated lungs were evaluated by flow cytometry (d). The number of spleen B10 cells (e), the serum cytokine levels (f) and splenic B cell FcγRIIb expression in offspring (g) from Alum-, OVA- or MOG-immunized (B10 cells only) or non-immunized mothers were also evaluated by flow cytometry at 3 days of age and after OVA immunization in the neonatal period at 20 days of age [20 (Im)]. A representative histogram of FcγRIIb expression in each group is shown. The data are presented as the mean ± SEM. *P≤0.05 compared with the respective offspring from non-immunized mothers

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