Fig. 6

Frequency of CTLA4⁺ T cells in atopic asthma patients inversely correlates with clinical severity. Representative FACS staining and summarized data for the frequency of a CD25⁺, b Foxp3⁺, c CTLA-4⁺, d Foxp3⁺CD25^hi and e Foxp3⁺CTLA-4⁺ in live CD4⁺ T cells. f PBMCs were stimulated with αCD3/αCD28 beads at a 1:1 bead:cell ratio for 5–6 h in the presence of GolgiPlug. Representative FACS staining (left) and summarized frequency of Foxp3⁺IL-10⁺ in CD4⁺ T cells after stimulation (right). Gating strategy based on isotype antibody staining profile. g Analysis of frequency of Foxp3⁺IL-10⁺ in CD4⁺ T cells from non-asthmatic subjects (NAS) and asthma patients (AS) treated with/without corticosteroid. h Cytokines in the supernatants of NAS and AS T cells cultured for 36 h in the presence of αCD3/αCD28-coated beads. i Correlation analysis of frequency of CTLA-4-expressing CD4⁺ T cells in asthma patients with ACT scores. j Correlation analysis of % predicted FEV1 and ACT scores. *P < 0.05. Errors bars indicate SD