Fig. 5

CD4⁺CD25⁺Foxp3⁺ regulatory T (Treg) cells were up-regulated with the treatment of artemisinin. a Flow cytometric analysis of CD4⁺CD25⁺Foxp3⁺ T cell subsets. Splenic mononuclear cells were collected from the spleen of the mice in different group and then performed by fluorescence-activated cell sorting analysis. Cells were first gated from CD4 positive portion and then analysis with CD25 and Foxp3. Upper right quadrant represented the CD4⁺CD25⁺Foxp3⁺ T cells, which were regulatory T cells. b The statistical analysis of (a). ***Indicated p < 0.001