Fig. 5

Effect of LPS and Rgs2 deficiency on cytokine and chemokine expression in the BAL fluid. Wild type or Rgs2^−/− mice were exposed to aerosolized PBS (20 ml) or LPS from E. coli 0127:B8 (20 ml at 150 μg/ml) for 30 min. BAL fluid was prepared 3, 6 or 24 h after LPS challenge, as indicated. Following centrifugation to remove cells, the supernatants were analyzed by Luminex. a Expression of those mediators showing LPS-induced expression of over 100 pg/ml in wild type animals is shown. Data from wild type animals (N = 6–8) is plotted in pg/ml as mean ± SE. Significance relative to PBS control was assessed by ANOVA with a Dunn’s post test. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. b Expression of cytokines and chemokines was compared between wild type (WT) and Rgs2^−/− (KO) animals 3 h following PBS exposure or 3 and 24 h following LPS exposure. Wild type, N = 6, 8 and 8 (respectively), or Rgs2^−/− 3, 8 and 8 (respectively). Significance was tested by Mann–Whitney U test. No significant differences between wild type or Rgs2^−/− were detected