A bronchoprotective role for Rgs2 in a murine model of lipopolysaccharide-induced airways inflammation

Table 1 Cytokines, chemokines and growth factors not detected or lowly expressed in the BAL fluid of LPS-exposed mice

A. Gene products not detected, or below 5 pg/ml, in BAL fluid
Gene products	IFNG, IL2, IL3, IL4, IL5, IL10, IL12 (p70), IL13, IL17, CXCL9

Gene product	PBS	3 h post-LPS		24 h post-LPS
Gene product	wt	wt	Rgs2 ^{− /−}	wt	Rgs2 ^{− /−}
B. Gene products showing low expression (< 100 pg/ml) in the BAL fluid following LPS exposure
CCL2 (MCP-1)	7.9 ± 1.4	10.9 ± 1.7	11.3 ± 4.1	6.6 ± 1.1	5.3 ± 0.8
CCL5 (RANTES)	4.6 ± 0.6	10.1*** ± 0.9	8.8 ± 0.7	5.7 ± 0.3	5.1 ± 0.5
CCL11 (Eotaxin)	2.5 ± 0.3	10.2*** ± 1.6	10.7 ± 1.2	4.3 ± 1.1	4.8 ± 0.98
CSF1 (MCSF)	13.4 ± 9.5	5.9 ± 4.4	4.9 ± 3.1	1.4 ± 0.1	1.5 ± 0.2
CXCL5 (LIX)	38.6 ± 21.7	32.5 ± 17.9	79.2 ± 45.2	10.5 ± 7.4	10.7 ± 7.4
CXCL10 (IP10)	1.9 ± 0.3	59.9*** ± 12.9	45.5 ± 5.5	6.6 ± 1.3	5.0 ± 1.2
IL1A	20.4 ± 5.1	30.4 ± 4.4	30.1 ± 2.5	17.9 ± 2.8	24.4 ± 4.2
IL1B	2.0 ± 0.4	6.0 ± 1.5	8.3 ± 1.3	2.6 ± 0.6	2.6 ± 0.5
IL7	4.9 ± 1.2	5.4 ± 0.4	6.8 ± 0.7	3.9 ± 0.4	4.8 ± 0.2
IL9	14.9 ± 5.8	12.1 ± 1.4	13.4 ± 1.5	18.2 ± 3.8	22.5 ± 4.1
IL12B (IL12 p40)	4.0 ± 1.0	8.6 ± 1.5	13.47^#± 1.6	5.4 ± 0.7	12.79^###± 2.5
IL15	3.5 ± 0.6	6.4 ± 1.2	5.2 ± 1.1	2.7 ± 0.5	2.5 ± 0.5
LIF	0.7 ± 0.1	15.4** ± 1.7	19.0 ± 1.1	0.9 ± 0.1	0.9 ± 0.1
VEGF	8.0 ± 1.8	8.0 ± 2.3	8.3 ± 0.7	11.0 ± 3.1	11.0 ± 1.6

As described for Fig. 5, wild type or Rgs2^−/− mice were exposed to aerosolized PBS (20 ml) or LPS from E. coli 0127:B8 (20 ml at 150 μg/ml) for 30 min. BAL fluid was prepared 3, 6 or 24 h after LPS challenge, as indicated. Following centrifugation to remove cells, the supernatants were analyzed by Luminex. Those mediators showing LPS-induced expression of < 5 pg/ml in wild type or Rgs2^−/− animals are listed in A. Mediators showing LPS-induced expression < 100 pg/ml in wild type or Rgs2^−/− animals are shown in B. Data (N = 6–8) are provided in pg/ml as mean ± SE. Significance between PBS control and the 3 and 24 h post-LPS exposure was assessed by nonparametric ANOVA with a Dunn’s post test. **P ≤ 0.01, ***P ≤ 0.001. Significance between wild type and Rgs2^−/− animals at each time post-LPS treatment was tested by Mann–Whitney U test. ^#P ≤ 0.05, ^###P ≤ 0.001

ISSN: 1710-1492