Table 2 Primer sequences used in real-time PCR
From: Frequency of CD4+ and CD8+ T cells in Iranian chronic rhinosinusitis patients
Gene | Size (bp) | Forward and reverse primers |
|---|---|---|
T-bet | 115 | F: 5′- CTGGAGGTGTCGGGGAAAC-3′ |
R: 5′-ATGGGAACATCCGCCGTCC-3′ | ||
GATA-3 | 107 | F: 5′-TCATTAAGCCCAAGCGAAGG-3′ |
R: 5′-GTCCCCATTGGCATTCCTC-3′ | ||
Ror-γt | 144 | F: 5′- AGACTCATCGCCAAAGCA -3′ |
R: 5′- CCTTGTAGAGTGGAGGGAAA -3′ | ||
FoxP-3 | 124 | F: 5′-ATTCCCAGAGTTCCTCCACAAC-3′ |
R: 5′-ATTGAGTGTCCGCTGCTTCTC-3′ | ||
β-Actin | 131 | F: 5′-TCCCTGGAGAAGAGCTACG-3′ |
R: 5′-GTAGTTTCGTGGATGCCACA-3′ |