Fig. 1

Phenotypic characteristics of peripheral blood classical (CD16⁻), intermediate and non-classical monocytes (CD16⁺), mDCs, and pDCs. Bivariate dot plot histograms illustrating the phenotypic strategy for the identification of the different monocyte subpopulations, pDCs and mDCs from peripheral blood. Classical monocytes (CD16⁻, blue events) express CD14 in the absence of CD16, they also show high reactivity for CD33, and HLA-DR; intermediate monocytes (CD16⁺, green events) are characterized as CD14-positive displaying an increasing positivity to CD16, together with positivity for CD33, and HLA-DR; non-classical monocytes (CD16⁺, orange events) are CD16-positive with a decreasing expression of CD14, presenting the lowest CD33 expression among monocytes subpopulations; mDCs (light blue events) are phenotypically characterized as negative for CD14, CD16, and they present lower SSC properties and higher expression of HLA-DR, CD33 and IgE bound to high affinity FcεRI compared to monocytes; pDCs are characterized by the high levels of CD123, but lower than basophils and high levels of HLA-DR expression