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Table 2 Effect of AZE, FP, and MP-AzeFlu on cytokine and sICAM-1 secretion from nasal mucosal epithelial cells

From: Superior effect of MP-AzeFlu than azelastine or fluticasone propionate alone on reducing inflammatory markers

Agent/dilution

Secretion, compared with FBS (%)

N

IL-6

IL-8

GM-CSF

sICAM-1

Control media

15.3 ± 2.1*

18.0 ± 2.7*

44.3 ± 4.7*

65.9 ± 5.1*

9

10% FBS

100

100

100

100

9

AZE

 1:102

76.1 ± 4.9*

90.1 ± 5.8

223.5 ± 34.9*

95.2 ± 2.3

9

 1:103

88.7 ± 4.3

87.0 ± 5.9

93.8 ± 6.0

92.8 ± 4.6

9

 1:104

84.6 ± 8.3

93.3 ± 3.3

89.7 ± 5.8

96.9 ± 1.8

9

 1:105

90.1 ± 3.8

91.0 ± 5.3

87.6 ± 3.8

98.9 ± 1.1

9

FP

 1:102

53.0 ± 4.9*

58.5 ± 2.3*

58.2 ± 5.9*

85.2 ± 3.5

9

 1:103

59.1 ± 5.4*

62.0 ± 5.7*

60.2 ± 5.8*

81.8 ± 5.4

9

 1:104

57.7 ± 6.6*

60.4 ± 3.6*

56.2 ± 6.5*

87.0 ± 3.4

9

 1:105

75.3 ± 6.9*

65.4 ± 5.9*

65.0 ± 7.2*

84.9 ± 7.3

9

MP-AzeFlu

 1:102

38.3 ± 4.2*

55.3 ± 3.4*

126.9 ± 9.8

84.0 ± 4.8

9

 1:103

55.0 ± 6.5*

60.8 ± 4.6*

61.7 ± 5.7*

86.3 ± 6.0

9

 1:104

52.9 ± 3.1*

60.8 ± 3.2*

57.9 ± 5.3*

92.7 ± 3.2

9

 1:105

72.4 ± 7.5*

65.1 ± 5.2*

69.7 ± 7.5*

88.9 ± 2.3

9

  1. Results are expressed as mean ± SEM. The Wilcoxon signed-rank test was used for analysis. *P < 0.05 compared with 10% FBS–induced secretion
  2. AZE azelastine hydrochloride, FBS fetal bovine serum, FP fluticasone propionate, GM-CSF granulocyte-macrophage colony-stimulating factor, IL interleukin, MP-AzeFlu intranasal AZE and intranasal FP in a single device, SEM standard error of the mean, sICAM-1 soluble intercellular adhesion molecule-1