Table 3 Fluorescently labelled antibodies for monocyte cell surface markers (all mouse anti-human antibodies except rat anti-human)
Epitope | Conjugate | Manufacturer | Clone | Isotype | Test volume (µl) |
|---|---|---|---|---|---|
CD45 | AF700 | Becton–Dickinson | HI30 | IgG1, k | 5 |
CD14 | BV510 | Becton–Dickinson | MφP9 | IgG2b, k | 5 |
CD16 | BV421 | Becton–Dickinson | 3G8 | IgG1, k | 3 |
CCR2 | AF647 | Becton–Dickinson | 48607 | IgG2b | 5 |
CX3CR1 (rat anti-human) | PE | MBL | 2A9-1 | IgG2b, k | 10 |