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Fig. 7 | Allergy, Asthma & Clinical Immunology

Fig. 7

From: Diagnosis of Vespa affinis venom allergy: use of immunochemical methods and a passive basophil activation test

Fig. 7

Gating strategy, evaluation of a patient serumand donor dependency of the passive BAT; a Basophils were gated in the lymphocyte region of the SSC/FSC pattern b selected high IgEpos population, c non-stimulated baseline control, d IgE control—stimulated with a different IgE source, e positive control (fMLP), f positive control (polyclonal anti IgE), g evaluation of %CD63 activation by V. affinis venom − 1 µg, hk basophils from two donors (donor 1 and 2) have been given a similar activation after incubation with sera from patient #5 and sera from patient #10

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