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Table 1 Expression of cytokines in bronchoalveolar lavage fluid (pg/ml)

From: Co-exposure to lipopolysaccharide and desert dust causes exacerbation of ovalbumin-induced allergic lung inflammation in mice via TLR4/MyD88-dependent and -independent pathways

Group

Animals (n)

IL-1β

IL-5

IL-6

IL-12

IL-13

IL-17A

WT mice

 Control

4

0.1 ± 0.1

1.1 ± 0.3

nd

14.4 ± 1.5

nd

nd

 LPS

4

4.5 ± 0.4

2.6 ± 0.8

17.8 ± 4.4

427.5 ± 74.6*

nd

nd

 H-ASD

4

5.0 ± 1.4

0.7 ± 0.1

nd

111.8 ± 10.2†

nd

nd

 OVA

5

5.0 ± 1.0

2.3 ± 0.8

nd

32.3 ± 4.4†

1.5 ± 0.3

nd

 OVA + LPS

5

9.4 ± 0.7

51.0 ± 12.8

9.8 ± 2.2

185.3 ± 30.9*†&

6.1 ± 1.8

5.0 ± 1.8

 OVA + H-ASD + LPS

5

12.5 ± 0.9*

113.0 ± 15.5*†§&#

31.1 ± 8.0*§&#

224.3 ± 49.4*†&

20.3 ± 3.7*†§&#

14.0 ± 3.2*†§&#

TLR2−/− mice

 Control

4

0.4 ± 0.4

0.7 ± 0.2

nd

18.6 ± 4.6

nd

nd

 LPS

4

2.7 ± 0.3

1.1 ± 0.3

14.4 ± 3.2

479.5 ± 39.0*

nd

nd

 H-ASD

4

4.5 ± 0.8

0.8 ± 0.2

nd

127.8 ± 22.4†

nd

nd

 OVA

5

3.7 ± 0.7

13.2 ± 10.0

0.5 ± 0.5

47.2 ± 17.9†

2.6 ± 2.0

nd

 OVA + LPS

5

24.5 ± 6.4*†&α

44.5 ± 12.2

28.0 ± 7.7*&α

327.6 ± 48.7*†&α

6.8 ± 1.7

1.2 ± 0.7

 OVA + H-ASD + LPS

5

15.0 ± 1.4*†§&

105.0 ± 19.9*†§&#

20.8 ± 6.3*§&

299.3 ± 39.2*†§&

14.8 ± 2.6*†§&#

13.4 ± 2.2*†§&#

TLR4−/− mice

 Control

4

0.2 ± 0.1

0.6 ± 0.2

nd

30.1 ± 2.5

nd

nd

 LPS

4

0.2 ± 0.2

1.7 ± 0.3

nd

22.1 ± 5.3

nd

nd

 H-ASD

4

3.7 ± 1.1

1.6 ± 0.2

0.8 ± 0.5

155.9 ± 15.3

nd

nd

 OVA

5

2.1 ± 0.1

3.7 ± 2.0

nd

24.4 ± 4.4

0.9 ± 0.3

nd

 OVA + LPS

5

2.6 ± 0.8β

2.5 ± 1.0

ndβ

25.4 ± 3.3αβ

1.6 ± 0.7

nd

 OVA + H-ASD + LPS

5

3.9 ± 0.8β

38.7 ± 25.7αβ

4.6 ± 2.9α

81.1 ± 21.3αβ

3.9 ± 2.4αβ

ndαβ

MyD88−/− mice

 Control

4

1.0 ± 0.3

0.2 ± 0.1

nd

5.0 ± 0.5

nd

nd

 LPS

4

1.4 ± 0.3

0.7 ± 0.4

nd

12.1 ± 1.9

nd

nd

 H-ASD

4

8.4 ± 3.8

1.3 ± 0.4

3.7 ± 3.1

42.4 ± 8.7

0.2 ± 0.2

nd

 OVA

5

7.2 ± 2.3

1.2 ± 0.1

nd

6.0 ± 0.6

1.1 ± 0.3

nd

 OVA + LPS

5

8.4 ± 2.5β

14.4 ± 12.5

ndβ

22.2 ± 2.1αβ

2.7 ± 1.6

nd

 OVA + H-ASD + LPS

5

6.0 ± 1.2

10.1 ± 5.3αβ

0.6 ± 0.6αβ

83.2 ± 7.8αβ

3.4 ± 1.6αβ

ndαβ

  1. Wild type (WT), toll like receptor (TLR)2−/−, TLR4−/−, myeloid differentiation primary response gene 88 (MyD88)−/−, four kinds of mouse were divided into six groups with 4–5 (n) which were treated intratracheally with saline (Control), lipopolysaccharide (LPS), heated-Asian sand dust (H-ASD), ovalbumin (OVA), OVA + LPS, OVA + H-ASD + LPS. Four times at 2-week intervals. All values were expressed as mean ± SEM
  2. nd not detected
  3. *p < 0.05 vs. control; †p < 0.05 vs. LPS; §p < 0.05 vs. H-ASD; &p < 0.05 vs. OVA; #p < 0.05 vs. OVA + LPS; αp < 0.05 vs. WT within the same group; βp < 0.05 vs. TLR2−/− within the same group
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Allergy, Asthma & Clinical Immunology

ISSN: 1710-1492

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