Co-exposure to lipopolysaccharide and desert dust causes exacerbation of ovalbumin-induced allergic lung inflammation in mice via TLR4/MyD88-dependent and -independent pathways

Ren, Yahao; Ichinose, Takamichi; He, Miao; Youshida, Seiichi; Nishikawa, Masataka; Sun, Guifan

doi:10.1186/s13223-019-0396-4

Table 2 Expression of chemokines in bronchoalveolar lavage fluid (pg/ml)

From: Co-exposure to lipopolysaccharide and desert dust causes exacerbation of ovalbumin-induced allergic lung inflammation in mice via TLR4/MyD88-dependent and -independent pathways

Group	Animals (n)	IFN-γ	KC	MCP-1	MCP-3	Eotaxin	TNF-α
WT mice
Control	4	nd	38.5 ± 8.6	nd	4.0 ± 0.5	1.4 ± 0.1	0.7 ± 0.1
LPS	4	nd	54.9 ± 10.4	13.2 ± 4.1	8.0 ± 1.4	5.5 ± 1.8	19.1 ± 5.4*
H-ASD	4	nd	183.6 ± 9.9*^†	48.4 ± 7.4	7.5 ± 0.3	1.5 ± 0.1	4.6 ± 0.3
OVA	5	0.4 ± 0.1	53.6 ± 5.9^§	1.8 ± 0.5	3.9 ± 0.6	1.3 ± 0.2	nd^†
OVA + LPS	5	1.5 ± 0.6	113.4 ± 15.1*^§&	26.4 ± 6.4	13.0 ± 1.8	16.1 ± 4.2*^†§&	16.2 ± 1.9*^&
OVA + H-ASD + LPS	5	1.9 ± 0.5*^†§	147.3 ± 25.2*^†&	129.8 ± 30.9*^†&#	54.3 ± 20.2*^†§&#	26.9 ± 4.4*^†§&#	20.1 ± 5.0*^§&
TLR2^−/− mice
Control	4	nd	57.1 ± 8.3	nd	3.7 ± 0.3	1.4 ± 0.1	0.7 ± 0.1
LPS	4	nd	81.3 ± 10.5	16.9 ± 3.2	7.5 ± 0.6	4.1 ± 0.6	12.7 ± 2.8
H-ASD	4	nd	162.0 ± 19.2*^†	54.2 ± 10.7	8.8 ± 0.6	1.5 ± 0.2	4.1 ± 0.7
OVA	5	0.5 ± 0.3	40.8 ± 20.6^§	1.5 ± 1.4	4.3 ± 1.0	1.1 ± 0.5	nd
OVA + LPS	5	3.1 ± 0.8*^†&	149.8 ± 12.4*^†&	36.9 ± 8.8	16.0 ± 1.3	14.9 ± 2.1*^†&	25.2 ± 6.0*^&
OVA + H-ASD + LPS	5	3.4 ± 0.9*^†§&	168.6 ± 12.1*^†&	134.4 ± 52.4*^†&#	53.9 ± 20.4*^†§&#	27.5 ± 4.5*^†§&#	29.7 ± 7.9*^†§&
TLR4^−/− mice
Control	4	nd	32.5 ± 4.1	nd	5.1 ± 0.3	1.9 ± 0.2	0.1 ± 0.1
LPS	4	nd	35.0 ± 3.9	1.5 ± 0.6	6.0 ± 0.2	2.04 ± 0.26	0.1 ± 0.1
H-ASD	4	nd	224.0 ± 20.8*^†	63.9 ± 7.3	7.8 ± 0.6	1.57 ± 0.17	5.1 ± 0.1
OVA	5	0.6 ± 0.1	38.3 ± 8.5^§	2.2 ± 1.1	3.9 ± 0.4	1.2 ± 0.3	nd
OVA + LPS	5	0.5 ± 0.1^β	42.6 ± 3.6^αβ	2.9 ± 0.6	5.6 ± 0.6	2.0 ± 0.2^αβ	nd
OVA + H-ASD + LPS	5	0.6 ± 0.3^β	80.0 ± 12.5^§αβ	43.5 ± 12.2^αβ	8.3 ± 1.3^αβ	4.3 ± 1.7^αβ	2.4 ± 1.2^αβ
MyD88^−/− mice
Control	4	nd	10.9 ± 1.0	nd	2.5 ± 0.3	1.0 ± 0.2	0.9 ± 0.1
LPS	4	nd	10.1 ± 0.6	0.7 ± 0.7	2.9 ± 0.3	1.04 ± 0.23	0.6 ± 0.1
H-ASD	4	0.1 ± 0.1	38.8 ± 12.0	33.3 ± 9.7	4.1 ± 0.6	1.59 ± 0.23	2.6 ± 0.3
OVA	5	0.8 ± 0.1	10.2 ± 0.8	0.9 ± 0.6	5.4 ± 0.8	0.7 ± 0.1	nd
OVA + LPS	5	0.7 ± 0.0^β	13.0 ± 1.6^αβ	5.3 ± 1.0	6.2 ± 0.5	2.1 ± 1.0^αβ	nd^αβ
OVA + H-ASD + LPS	5	1.0 ± 0.1^β	24.5 ± 1.6^αβγ	57.9 ± 9.7	6.9 ± 1.2^αβ	2.3 ± 0.4^αβ	nd^αβ

Wild type (WT), toll like receptor (TLR)2^−/−, TLR4^−/−, myeloid differentiation primary response gene 88 (MyD88)^−/−, four kinds of mouse were divided into six groups with 4–5 (n) which were treated intratracheally with saline (Control), lipopolysaccharide (LPS), heated-Asian sand dust (H-ASD), ovalbumin (OVA), OVA + LPS, OVA + H-ASD + LPS. Four times at 2-week intervals. All values were expressed as mean ± SEM
nd not detected
*p < 0.05 vs. control; ^†p < 0.05 vs. LPS; ^§p < 0.05 vs. H-ASD; ^&p < 0.05 vs. OVA; ^#p < 0.05 vs. OVA + LPS; ^αp < 0.05 vs. WT within the same group; ^βp < 0.05 vs. TLR2^−/− within the same group; ^γp < 0.05 vs. TLR4^−/− within the same group

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