Fig. 3

Effect of inhibitors of TMEM16A and PI3K on EGF-induced MUC5AC expression in HNECs from CRSwNP. Cells were treated with EGF (100 ng/ml) alone or with EGF following pretreatment for 30 min with T16Ainh-A01 (10 μM; the specific TMEM16A inhibitor) or with LY294002 (25 μM; the PI3K inhibitor). a After 4 h total RNA was analyzed by real-time RT-PCR for MUC5AC mRNA expression (n = 6); and b after 24 h the MUC5AC protein in lysates was detected by ELISA (n = 6). *P < 0.05, between two groups