Fig. 5

Effect of inhibitors of TMEM16A and PI3K on EGF-induced TMEM16A and MUC5AC expression in HNECs from CRSwNP. Cells were treated with EGF (100 ng/ml) alone or with EGF following pretreatment for 30 min with T16Ainh-A01 (10 μM; the specific TMEM16A inhibitor) or with LY294002 (25 μM; the PI3K inhibitor). After 24 h the cells were stained for TMEM16A and MUC5AC by immunofluorescence staining. The number of TMEM16A-positive cells, MUC5AC-positive cells, and cells coexpressing TMEM16A and MUC5AC was quantified and expressed at a percentage of total cells. Traces a–f show representative confocal images of control cells (a), EGF-treated cells (b), EGF-treated cells pretreated with T16Ainh-A01 (c), cells treated with T16Ainh-A01 (d), EGF-treated cells pretreated with LY294002 (e), cells treated with LY294002 (f). TMEM16A (green), MUC5AC (red) and nuclei (blue) are demonstrated (n = 4). Scale bar = 20 μm. *P < 0.05, vs control, #P < 0.05, vs EGF