Fig. 3

Detection of a clonally expanded CD4⁺CD8⁺ T cell population in blister fluid and peripheral blood. a CD4⁺CD8⁺ T cell frequencies within blister fluid (BF) and peripheral blood (PB) in two different flow cytometry staining panels (panel 1 and panel 2) 3–5 days after the initiation of corticosteroid (CS) treatment. b Frequencies of CD4⁺CD8⁺ T cells within PB 2 years prior to onset of disease, during the acute phase before initiation of CS treatment and 19 weeks after the acute stage. c Flow cytometry analysis of the frequency of TCRVβ2⁺ cells among CD4⁺CD8⁻, CD4⁻CD8 + and CD4⁺CD8⁺ T cell subsets at day 5 after initiation of CS treatment. Antibody against TCRVβ2 was labeled to FITC and PE at equal amounts. d Flow cytometry analysis of the frequency of PB T cells expressing the cytotoxic mediators granulysin and perforin among CD4⁺CD8⁻, CD4⁻CD8⁺ and CD4⁺CD8⁺ T cell subsets, assessed 10 days after initiation of CS treatment. The most relevant findings are highlighted in red