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Fig. 2 | Allergy, Asthma & Clinical Immunology

Fig. 2

From: Trained immunity induced by in vivo peptide-based STAT6 inhibition prevents ragweed allergy in mice

Fig. 2

Adoptively transferred STAT6-IP CD11c+ DCs prevented allergic airways disease (AAD) in naïve recipient mice. BALB/c mice were vaccinated with STAT6-IP or STAT6-CP as described in methods. a CD11c+ DCs were then isolated from STAT6-IP (IP-DCs cells) or STAT6-CP (CP-DCs) vaccinated mice and adoptively transferred intratracheally into naïve BALB/c mice. Twenty-four hours later recipient mice were sensitized and challenged with ragweed as per protocol. b Bronchial airway hyperresponsiveness (AHR) to methacholine following challenge with ragweed was assessed in recipient mice using the Flexivent small animal ventilator. PBS (filled circle), Ragweed (filled square), IP-T cells (filled upward triangle), and CP-T cells (filled downward triangle), respectively. c Differential cell counts from recipient mice were obtained from recovered bronchial alveolar lavage (BAL) fluid and stained with Diff-Quick method. White column denotes macrophages (open square), lymphocytes (filled square) light grey, neutrophils (filled square) dark grey, and eosinophils (filled square) black. d Serum OVA-specific-IgE from recipient animals determined by ELISA. Splenocytes from STAT6-IP, -CP or sham vaccinated mice recipient mice were cultured in triplicates in the presence of ragweed for four days and e IL-4, f IL-10, g IL-13, h IFNγ and i TGFβ were quantified from supernatants by ELISA. j Inflammatory genes (Muc-2, Muc-5ac, Eotaxin-1) were investigated in whole lung homogenates from mice using RT-PCR. k IDO expression was assessed by RT-PCR in lung cells of vaccinated mice. l The frequency of T regulatory (CD4+CD25+FOXP3+) cells was investigated in splenocytes from vaccinated mice determined by flow cytometry analysis. Each experiment included 6–8 animals per group and experiment replicated at least three times. Error bars indicated the standard error of mean. IP group compared with ragweed group, **p < 0.01, and ***p < 0.001 were considered significant

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