Fig. 3
From: Trained immunity induced by in vivo peptide-based STAT6 inhibition prevents ragweed allergy in mice
CD4+ T cells from IP vaccinated DCs or T cells mice suppressed the proliferation of ragweed sensitized T cells. CSFC stained CD4+ T cells, purified from spleens of ragweed sensitized mice, were mixed with CD4+ T cells, isolated from IP-T, IP-DC treated or sham control mice and cocultured in 24-well plates. Cells were stimulated with 10 mg/mL ragweed in the presence of 2 × 105 purified CD11c+ DCs from allergen naïve mice. After 3 days, cells were harvested, and CFSE dilution was analyzed by means of flow cytometry. PBS control responder cells were derived from non-sensitized mice. Cells from IP-T and IP-DC recipient animals inhibited ragweed-mediated proliferation of T cells. Each experiment included 6–8 animals per group and experiment replicated at least three times. Error bars indicated the standard error of mean. IP group compared with ragweed group, ***p < 0.001 were considered significant