Fig. 2

Human interleukin 4 (hIL-4) neutralization by single-chain variable fragment (scFv) on 2D models. a hIL-4 neutralization by scFv and Ab on HEK-Blue™ IL-4/IL-13 cells. Optical densities at 620 nm (OD 620 nm) were determined and results were expressed in arbitrary units after normalization to the maximum value (100%) measured in cells stimulated with 10 ng/ml hIL-4. In each experiment and for each condition, cells were seeded in triplicates (n = 4 for 1, 10 and 10 nM doses; n = 3 for 200 nM dose). Results are expressed as mean ± SEM. b Human interleukin 8 (hIL-8) quantification in supernatants of normal human keratinocytes (NHKs) stimulated with hIL-4 ± polyinosinic-polycytidylic acid (poly I:C) (25 ng/ml and 2 µg/ml, respectively) and treated with anti-hIL4 scFv at different concentrations for 24 h (n = 4). For the four donors, experiments were performed in duplicates. Results are normalized in relation to the quantity of total proteins per well and expressed as mean ± SEM (NS non-significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001)