BMI trajectory in childhood is associated with asthma incidence at young adulthood mediated by DNA methylation

Table 2 Effects of childhood BMI trajectories on asthma incidence in adulthood via pre-adolescence DNAm

CpG sites	IoW^a				ALSPAC^b				Genes	Gene location	Chr.^e
	Indirect eff.^c	P-value	Direct eff.^d	P-value	Indirect eff.^c	P-value	Direct eff.^d	P-value
cg23632109	0.27	0.03	0.06	0.89	0.02	0.92	0.53	0.37	TBC1D16	5′UTR, 3′UTR	17
cg03584646	− 0.28	0.04	1.01	0.01	− 0.09	0.60	0.64	0.48	TBC1D8	Body, 3′UTR	2
cg03508767	− 0.25	0.04	0.99	0.01	− 0.36	0.23	0.83	0.31	RASA2	3′UTR	3
cg10817500	0.32	0.02	0.39	0.34	0.09	0.87	0.48	0.65	SSH1	5′UTR, 1st Exon,	12

The first CpG is for males and the remaining three CpGs are for females. The indirect effects of CpGs in IoW and ALSPAC cohort in the same direction are highlighted in bold font.
^aFor the analyses in IoW, the path analyses were adjusted for second-hand smoking status at 1, 2 and 4 years, socio-economic status (SES), active smoking status at 18 years, age of pubertal events (age at onset of voice deepening for males and age at onset of menarche for females)
^bAnalyses of ALSPAC used similar covariates: active smoking status at 17 years, socio-economic status (SES) and pubertal events
^cIndirect eff.: estimates of indirect effects
^dDirect eff.: estimates of direct effects
^eChr.: Chromosome