Fig. 2

Serum IgE reactivity against purified jellyfish collagen and five types of edible jellyfish extracts. Each well was coated with purified jellyfish collagen solution and five kinds of acid-soluble jellyfish extracts, and serum from the patient or 3 control patients was applied to the appropriate wells for the reaction. The serum jellyfish-specific IgE antibody bound to the coated jellyfish antigen was recognized with an HRP-conjugated mouse anti-human IgE Fc receptor monoclonal antibody. This photo shows the color change to blue in each well due to TMB substrate oxidized by enzymatic reaction. The graphs indicate the absorbance at 450 nm after the color change to yellow after the addition of hydrochloric acid