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Fig. 7 | Allergy, Asthma & Clinical Immunology

Fig. 7

From: Flow cytometry optimizing the diagnostic approach in inborn errors of immunity: experience from Egypt

Fig. 7

Histograms showing the gating strategy using 10 different monoclonals on the 8-color FACs CANTO-II for analysis of different lymphocytes subsets in a patient underwent HSCT. Lymphocytes are characterized by CD45 expression, forward and side scatter. APC-CD3 blotted against PE-CD56 for NK assessment. APC-CD3 blotted against PE-cy 7-CD19 for T and B cells identification. For T cytotoxic and T helper, APC-CD3 is blotted against FITC-CD8 and PerCP-Cy 5.5-CD4 respectively. Gating on CD4+, naïve and memory T helper are assessed by BV510-CD45RA and PE-CD45RO. Gating on CD19+, naïve and memory B cells are assessed by FITC-IgD and BV421-CD27

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Allergy, Asthma & Clinical Immunology

ISSN: 1710-1492

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