Fig. 2

HDM decreased KIF2A expression in epithelial cells. A 16-HBE were left untreated or treated with different concentrations of HDM for 24 h. The expression of KIF2A was measured with Western blotting and the density quantification of KIF2A was expressed as a ratio relative to β-actin (B) MLE-12 cells were left untreated or treated with different concentrations of HDM for 24 hous. The expression of KIF2A was measured by qPCR. C MLE-12 cells were left untreated or treated with HDM as described in (B), and the KIF2A expression levels were examined using a Western blot assay. The density quantification of KIF2A was expressed as a ratio relative to β-actin (D) MLE-12 cells were left untreated or treated with HDM (50 μg/mL) for the indicated time periods; then, the KIF2A expression levels were measured with Western blotting and the density quantification of KIF2A was expressed as a ratio relative to β-actin (E) Immunofluorescence staining of SP-C (alveolar epithelial cell marker) in primary ATII cells. Scale bar, 100 μm. F Primary epithelial cells were left untreated or treated with HDM (50 μg/mL) for the indicated time periods; then, the expression of KIF2A was measured using western blotting. The density quantification of KIF2A was expressed as a ratio relative to β-actin. Data are presented as mean ± SEM of independent experiments with similar results (n = 3 ~ 8). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001