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Fig. 4 | Allergy, Asthma & Clinical Immunology

Fig. 4

From: KIF2A decreases IL-33 production and attenuates allergic asthmatic inflammation

Fig. 4

HDM mediated IL-33 production in epithelial cells by autophagy. A MLE-12 cells were left untreated or treated with different concentrations of HDM for 24 h, and the autophagy levels (LC3B and ATG5) were examined using a Western blot assay. The density quantification of LC3B and ATG5 was expressed as a ratio relative to β-actin. B MLE-12 cells were left untreated or treated with HDM extract (50 μg/mL) for the indicated time periods; then, autophagy levels were measured with Western blotting. The density quantification of LC3B and ATG5 was expressed as a ratio relative to β-actin (C) Representative immunofluorescence images showing increased autophagy levels after HDM treatment in MLE-12 cells. Scale bar, 5 μm. D MLE-12 cells were treated with HDM (50 μg/mL) alone or pretreated with autophinib. The expression levels of IL-25, TSLP, and IL-33 were measured with qPCR. E MLE-12 cells were treated with HDM (50 μg/mL) alone or pretreated with autophinib. The expression levels of autophagy and IL-33 were measured using a western blot assay and the density quantification of IL-33 was expressed as a ratio relative to β-actin. Data are presented as mean ± SEM of independent experiments with similar results (n = 3 ~ 6). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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