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Fig. 6 | Allergy, Asthma & Clinical Immunology

Fig. 6

From: KIF2A decreases IL-33 production and attenuates allergic asthmatic inflammation

Fig. 6

KIF2A regulated HDM-induced autophagy via mTORC1 signaling. A MLE-12 cells were left untreated or treated with HDM extract (50 μg/mL) for the indicated time periods; then, the activation status of mTORC1 was determined with Western blotting and quantification was based on band density. B MLE-12 cells were transfected with pCMV6-KIF2A or vehicle and then treated with HDM (50 μg/mL) 36 h after transfection. The activation status of mTORC1 was examined 2 h after HDM exposure with Western blotting and quantification was based on band density. C MLE-12 cells were treated with HDM (50 μg/mL) alone or pretreated with ( +) Usnicacid. The expression levels of autophagy and phosphorylation of P70S6K1 at Thr 389 were measured using a Western blot assay and and quantification was based on band density. Data are presented as mean ± SEM of independent experiments with similar results (n = 4). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001

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