Fig. 7

KIF2A attenuated allergic airway inflammation. A Experimental design of the study. B The level of KIF2A in different groups was examined using qPCR. C Immunohistochemical staining for KIF2A in lung sections from mice in different groups and AOD was measured with imageJ. D Mice inhaled increasing doses of acetylcholine (0–100 mg/mL), and AHR was measured. E The concentration of total IgE in seurm was measured with ELISA. F The concentrations of different cytokines (IL-4, IL-5, IL-13, Eotaxin and IFN-γ) in lung homogenates were measured with ELISA. G Total and differential cell counts in bronchoalveolar lavage fluid were determined by Wright-Giemsa staining. H Lung sections were stained with hematoxylin and eosin to analyze the infiltration of inflammatory cells, and the severity of peribronchial inflammation was graded semiquantitatively. I Lung sections were stained with PAS to assess goblet cell hyperplasia, PAS-positive and PAS-negative epithelial cells were counted, and the percentage of PAS-positive cells per bronchiole was calculated. Scale bar, 50 μm. Data are presented as mean ± SEM of independent experiments with similar results (n = 6 ~ 10). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001