Fig. 4

Expression of PAR1 in HAE patient and control. Samples were incubated with rabbit anti-PAR1 antibody followed by peroxidase conjugated goat anti-rabbit secondary antibody. The immune reaction was developed by DAB and counterstained with hematoxylin. HAE patient’s (A, C, E) and control’s (B, D, F) epiglottis (A, B), true vocal cord (C, D) and false vocal cord (E, F) regions are shown. Black arrows indicate endothelial cells and white arrows indicate epithelial cells. Scale bar: 50 μm. Expression pattern of PAR1 in different anatomical locations is compared as staining intensity scores. Green bars represent the control subject’s values, red bars represent the HAE patient’s values. Sum scores (I) were calculated by the summation of epithelial cells (G), endothelial cells (H), leukocytes and interstitial space staining intensity scores (individually ranged from 0 to 3). (EPI: epiglottis, TVC: true vocal cord, FVC: false vocal cord.)