Fig. 3

FHL1 down-regulation alleviates airway inflammation in mice with asthma-like airway inflammation. Normal mice served as the control and asthmatic mice were treated with lentivirus harboring oe-FHL1/oe-NC or sh-FHL1/sh-NC. A Expression of FHL1 in lung tissues of asthmatic mice determined by RT-qPCR. B, The airway resistance in asthmatic mice on the 1st day. C The airway resistance in asthmatic mice at various time points in response to Mch at a dosage of 200 mg/mL. D The airway responsiveness in asthmatic mice. E The number of total cells and percentage of eosinophils in BALF of asthmatic mice. F Levels of IL-4, IL-5, IL-13 and IFN-γ in BALF supernatant of asthmatic mice measured by ELISA. G HE staining images of lung tissues of asthmatic mice. H Masson’s trichrome staining images of lung tissues and collagen deposition area of asthmatic mice. * p < 0.05 compared with normal mice. # p < 0.05 compared with asthmatic mice treated with lentivirus harboring sh-NC. $ p < 0.05 compared with asthmatic mice treated with lentivirus harboring oe-NC. n = 10 mice for each treatment