Fig. 4

Knockdown of FHL1 induces inhibition of ASMC proliferation and promotion of their apoptosis in vitro. ASMCs isolated from normal mice remained non-transduced while ASMCs from asthmatic mice were transduced with oe-FHL1/oe-NC or sh-FHL1/sh-NC. A ASMCs from asthmatic mice identified by immunofluorescence. Morphological characteristics (a) and fluorescence microscopic observation (b) of ASMCs. B FHL1 mRNA expression in ASMCs determined by RT-qPCR. C, ASMC viability assessed by MTT assay. D Protein levels of cleaved PARP and cleaved caspase-3 in ASMCs measured by Western blot analysis. E Flow cytometric data showing cell cycle distribution. F Flow cytometric data showing apoptosis of ASMCs. * p < 0.05 compared with ASMCs from normal mice. # p < 0.05 compared with asthmatic mice-derived ASMCs transfected with sh-NC. $ p < 0.05 compared with asthmatic mice-derived ASMCs transfected with oe-NC. Cell experiments were performed in triplicate