Fig. 6

Silencing of FHL1 blocks the MAPK pathway in vivo. Normal mice served as the control and asthmatic mice were treated with lentivirus harboring oe-FHL1/oe-NC or sh-FHL1/sh-NC. A Schematic diagram of the binding of MAPK pathway protein ERK to the LIM domain of FHL1, which is composed of four LIM domains (lim1-4) and an additional N-terminal half LIM domain (z). B mRNA levels of FHL1, ERK, p38MAPK and JNK in lung tissues of asthmatic mice determined by RT-qPCR. C Protein levels of FHL1, ratio of p-ERK/ERK, ratio of p-p38MAPK/p38MAPK, and ratio of p-JNK/JNK in lung tissues of asthmatic mice measured by Western blot analysis. D Immunohistochemical analysis of protein expression of p-ERK, p-p38MAPK, and p-JNK in lung tissues of asthmatic mice. n = 10 mice for each treatment. * p < 0.05 compared with normal mice. # p < 0.05 compared with asthmatic mice treated with lentivirus harboring sh-NC. $ p < 0.05 compared with asthmatic mice treated with lentivirus harboring oe-NC