Figure 4

Impaired allergic asthmatic pulmonary Treg function is associated with elevated airway TSLP. A. TSLP expression in BAL of AA, HC, and NA subjects (n = 15). B. Correlation of TLSP levels in BAL of AA subjects with IL-10 production and suppression activity of AA pulmonary Treg (n = 13). Straight lines represented best-fitted lines. Dotted lines represented 95% confident intervals. C. (Top) Effects of blocking TLSP-R antibodies on AA BAL-mediated inhibition of IL-10 production by HC pulmonary Treg (n = 5). (Bottom) Representative FACS plots of IL-10 production by HC pulmonary Treg in different stimulatory conditions. D. Effects of NA BAL on IL-10 production by HC pulmonary Treg (n = 5). Data represented intracellular flow cytometric and ELISA results. Kruskal Wallis tests (A), Spearman tests (B) and Wilcoxon tests (C) were used for statistical analysis. Horizontal bars represented median values as indicated throughout the figure.