Table 1 Quantitative PCR primer sequences
Gene | NCBI accession | Forward (5′ → 3′) | Reverse (5′ → 3′) | CDS region†| Product size |
|---|---|---|---|---|---|
GAPDH | NM_002046.4 | GCACCGTCAAGGCTGAGAACGG | CGACGTACTCAGCGCCAGCATC | c.173-286 | 114 |
PPIA | NM_021130 | TAAAGCATACGGGTCCTGGCATCT | ATCCAACCACTCAGTCTTGGCAGT | c.269-369 | 101 |
ACTB | NM_001101.3 | GTTGCGTTACACCCTTTCTT | ACCTTCACCGTTCCAGTTT | c.*16-*162 | 147 |
DDIT3 | NM_001195053.1 | GAAATGAAGAGGAAGAATCA | TTCTCCTTCATGCGCT | c.197-437 | 241 |
XBP-1s | NM_001079539.1 | ATGGATGCCCTGGTTGCTGAAGA | TGCACCTGCTGCGGACTCA | c.415-504 | 90 |
XBP-1u | NM_005080.3 | AGCACTCAGACTACGTGCACCTCT | CCAGAATGCCCAACAGGATATCAG | c.495-624 | 130 |
ORMDL1 | NM_016467.4 | AATGGCTGGTCCTTCAAGTGCT | ACCCTCACTGTGATGCCCTTTA | c.*121-*269 | 149 |
ORMDL2 | NM_014182.4 | ACACACTGGGAGCAAATGGACT | AGTGCGCAGCATCATACTTGGT | c.250-370 | 121 |
ORMDL3 | NM_139280.2 | TCAGGCAGCCAAAGCACTTTAACC | ACCCATCCCACACTTGCTTCCATA | c.*358-*496 | 139 |
BCL6 | NM_001706.4 | ACAATCCCAGAAGAGGCACGAAGT | GCTCGAAATGCAGGGCAATCTCAT | c.790-952 | 163 |
CCL2 | NM_002982.3 | TCGCTCAGCCAGATGCAATCAATG | TGGAATCCTGAACCCACTTCTGCT | c.65-259 | 195 |
CCL5 | NM_002985.2 | TGCCTGTTTCTGCTTGCTCTTGTC | TGTGGTAGAATCTGGGCCCTTCAA | c.*36-*127 | 92 |
CSF2 | NM_000758.3 | AAATGTTTGACCTCCAGGAGCCGA | GGTGATAATCTGGGTTGCACAGGA | c.185-357 | 173 |
IL12A | NM_000882.3 | ATGATGGCCCTGTGCCTTAGTAGT | AGGGCCTGCATCAGCTCATCAATA | c.457-611 | 155 |
IL13RA1 | NM_001560.2 | GTCCCAGTGTAGCACCAATGA | CAGTCACAGCAGACTCAGGAT | c.297-391 | 95 |
ADRB2 | NM_000024.5 | TCATCATGGGCACTTTCACCCTCT | AGCTCCTGGAAGGCAATCCTGAAA | c.830-1016 | 187 |
VEGFA | NM_001025366.2 | TTCAGGACATTGCTGTGCTTTGGG | TGGGCTGCTTCTTCCAACAATGTG | c.*778-*969 | 192 |
IL23A | NM_016584.2 | TCGGTGAACAACTGAGGGAACCAA | TGGAATCTCTGCCCACTTCCACTT | c.-140- -54 | 87 |