Figure 2

Downregulation Rho kinase RGS-5 and MMP10 but upregulation of MMP9 and MMP28 genes in KO lung. Real time PCR analysis was used to quantitate expression of mRNA for the particular genes as calculated by relative index of Ct values normalized to GAPDH by real time PCR. PCR was carried out using the comparative Ct method (Applied Biosystems software) with SYBR Green PCRcore reagents (Applied Biosystems) and anlysed using Applied Biosystems 7900HT Real-Time PCR System software SDS 2.2.1. All primers used were specific to mouse. * denotes p value<0.01 compared to WT+OVA values. # denotes p value<0.01 compared to WT+alum (control baseline values). n=5/group pooled from 2 experiments. Expression of the gene of interest was expressed in relative values normalized to the values obtained for mouse GAPDH. Compared to post-OVA WT values, Rho kinase RGS-5 mRNA was decreased 2.3-folds and MMp-10 3-fold, while MMP-9 was increased 8-fold and MMP-28 increased 2.3-fold (*denotes p value<0.05 compared to post-OVA WT).